Investigate an agricultural biotechnology lab process.

Investigation may include

• DNA extraction
• polymerase chain reaction (PCR)
• gel electrophoresis
• spectrophotometry
• creation of transgenic organisms
• gene knockout technology
• clustered regularly interspaced short palindromic repeats (CRISPR) system for gene editing
• transgene-free gene editing
• use of somatic cell nuclear transfer (i.e., cloning).

Process/Skill Questions:

• What steps are taken to plan and develop a plant field trial?
• Why is it important to conduct and collect data from field trials?
• What can be learned from analyzing data collected from a field trial?
• What is the difference between cloning from embryos and cloning from differentiated cells?
• What are the steps that ensure safety throughout this process?